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Rachel Schecter – BenchFly http://www.benchfly.com/blog The Premier Video Platform for Scientists Tue, 09 Jan 2018 23:04:23 +0000 en-US hourly 1 https://wordpress.org/?v=4.9.3 PCR Tube Strip Labeling Tip http://www.benchfly.com/blog/pcr-tube-strip-labeling-tip/ http://www.benchfly.com/blog/pcr-tube-strip-labeling-tip/#respond Mon, 19 Oct 2009 04:46:57 +0000 http://www.benchfly.com/blog/?p=2279 The very first things I ordered when I officially joined my lab were multi-colored Sharpies and a rainbow of lab tape.  The color gives me a little bit of joy in a world of black, white, grey, and beige.  Yes, I am the kind of person who wants tube racks in every different color.  Yes, I am the kind of person who gets excited to use multicolored eppis.  Yes, I am the kind of person who enjoys adding NaOH to phenol red solutions just to watch the yellow contrast with the bright pink.

Yes, I am the kind of person to realize this sounds stupid, but color also serves me as an organization system.  I can quickly identify what I need even when my life is not the most organized (which, uh, it usually isn’t) and when I am in a hurry (which, uh, I usually am).  If I need to grab my P200, I don’t need to waste precious seconds picking up each pipet and reading the labels, I just grab the green one.  When looking through 81 tubes in the freezer box to find anti-MAP, I know it has a purple label, because all of my primary antibodies have purple labels, while secondaries have red labels.

Along with my desire to make everything technicolor, I also look for ways to simplify my life where ever possible.  Anyone who has ever PCR’d before is familiar with those teeny tiny tubes and how difficult they are to label.   You could use the numbers on the tubes themselves, but, oh yeah, they are cloudy-clear on a clear background in a font size that wouldn’t even register in a word processing program, which isn’t exactly ideal for legibility.  The lids are off limits as they get removed, and any writing on the parts of the tube that make contact with the block is susceptible to being rubbed off.  That leaves you with a space a few square millimeters; even using finest Sharpies with the most immaculate handwriting, writing more than a couple of letters introduces ambiguity.  So I applied my favorite lab tool – color – to the problem, and now labeling my PCR strips requires the fine motor skills of a toddler.  Assuming you can also scribble, you’ll never have to struggle with labeling them again!

WATCH VIDEO!

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Polypropylene vs Polystyrene http://www.benchfly.com/blog/polypropylene-vs-polystyrene/ http://www.benchfly.com/blog/polypropylene-vs-polystyrene/#comments Mon, 24 Aug 2009 05:00:54 +0000 http://www.benchfly.com/blog/?p=1016 I sometimes feel like there is only one way to learn anything in lab:  the hard way.  A lot of little details in lab go unmentioned, yet can make or break an experiment, and you won’t know it until things either don’t work, or go horribly awry.  Losing a day and a half because you didn’t realize that all plastic is not created equal falls into the latter category.  Losing someone else’s day and a half is, well, infinitely worse.

The easiest way to avoid the scorn of the entire lab is to carefully check what everyone is currently using and just stick with one kind.  However, if that is not possible or, say, you already ordered polystyrene tubes for the lab when polypropylene is the preferred kind and no one can tell them apart, yikes.  Unsurprisingly, I learned this the hard way; a post-doc in the lab was horrified to open the centrifuge on maxipreps to find the conical bottomed tubes fractured and his samples all over the rotor.  It was a stupid mistake that I thought only I was capable of making, until a month later when a tech from a brand new lab stopped by to ask what kind of conical bottom tubes we used, and asked very specifically about maxipreps.  I laughed because I knew instantly what the problem was.  I explained how you could hold them up to the light to determine whether the plastic was clear or cloudy, but that’s hard to determine without the other kind of tube as a basis for comparison.  That’s when a hammer and the desire to destroy things comes in handy.

Polyprop vs polysty

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