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How to Perform Colony PCR

By Alan M. 25752 Views
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Colony PCR is an easy way to rapidly screen ligation reactions for positive clones - and will save you both time and money. Once you try it, this technique will change your molecular biology life.

100x20 cell culture dish

Supplier: BD Biosciences
Catalog Number: 353003

200uL TipOne yellow tip wafers

Supplier: USA Scientific
Catalog Number: 1111-0706

Ampicillin, sodium salt

Supplier: SigmaAldrich
Catalog Number: A9518-5G

FailSafe PCR 2X PreMix, D

Supplier: Epicentre
Catalog Number: FSP995D

P20 Pipetman

Supplier: Gilson
Catalog Number: F123600

P200 Pipetman

Supplier: Gilson
Catalog Number: F123601

PCR 8-well Tube strips and caps

Supplier: VWR
Catalog Number: 20170-004

Round-bottom culture tubes, 15mL

Supplier: BD Biosciences
Catalog Number: 352059

SealRite eppi tubes, 1.5mL

Supplier: USA Scientific
Catalog Number: 1615-5500

Taq DNA Polymerase

Supplier: New England Biolabs
Catalog Number: M0273S

UltraPure Agarose

Supplier: Invitrogen
Catalog Number: 15510-027

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