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Western Blot Transfer, Wet Apparatus

By Brenda C 12239 Views
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Using a wet transfer apparatus for western blots is easy as long as you pay attention to a few key steps as shown here.

7.5% Tris-HCl Ready Gel

Supplier: BioRad
Catalog Number: 161-1154

Fiber Pads

Supplier: BioRad
Catalog Number: 170-3933

Mini Trans Blot Module

Supplier: BioRad
Catalog Number: 170-3935

MiniProtean Tetra cell

Supplier: BioRad
Catalog Number: 165-8005

Pre-cut Nitrocellulose, 0.2um pore size

Supplier: BioRad
Catalog Number: 162-0146

Precision Plus Protein Kaleidoscope Mol Weight Standards

Supplier: BioRad
Catalog Number: 161-0375

Pyrex glass drying tray, 200mL

Supplier: Corning
Catalog Number: 3175-8

Thick Blot Paper

Supplier: BioRad
Catalog Number: 170-3932

I use two pieces of nitrocellulose per gel as a safeguard against overtransferring small bands, but you can also just use one if you prefer.

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