BenchFly
  • Join
  • Login
  • Facebook
  • Twitter
  • RSS
  • What is BenchFly?
  • Video Protocols
    • Biology
    • Chemistry
    • DNA/RNA
    • Instruments and Equipment
    • Other
    • Protein
    • Proteomics
    • Useful Resources
  • Productions
  • Blog
  • About

Tissue Prep for LCMS - Pt.8: Final Desalting

By Ayers Institute 4923 Views
Tweet

Add Your Own

Share Your Own Video Like: Tissue Prep for LCMS - Pt.8: Final Desalting

Sponsor

  • Summary
  • Products
  • Attachments
  • Notes

Peptide desalting is performed on the purified, digested sample in the final step of preparing the tissue sample for LCMS analysis.

Acetonitrile, HPLC Grade

Supplier: Fisher Scientific
Catalog Number: A998-1

Nunc deep well plates, 2mL sterile

Supplier: Nunc
Catalog Number: 278743

Oasis HLB 96-well plate uElution Plate 30um

Supplier: Waters
Catalog Number: 1860001828BA

Oasis HLB 96-well plate, 30um (10mg)

Supplier: Waters
Catalog Number: 186000128

Oasis HLB 96-well, 30um (5mg)

Supplier: Waters
Catalog Number: 186000309

Trifluoroaceticacid, 99.0% assay

Supplier: Fisher Scientific
Catalog Number: 04902-100

Water, HPLC Grade

Supplier: VWR
Catalog Number: JT9823-1

Adobe PDF File Text Version of “Tissue Prep for LCMS - Pt.8: Final Desalting”

See the attached file for the text version of the protocol and check out the other videos in the Tissue Prep for LCMS series.

Comments on “Login”

Send Us Your Feedback!

Have a suggestion for the “Tissue Prep for LCMS - Pt.8: Final Desalting” video? Contact us with your suggestions.

Popular video protocols!

Almost done...

A new approach to peer review for scientific publishing - how you can publish faster

Play Video
Almost done...

Opening a Locked Eppendorf Centrifuge

Play Video
Almost done...

How to Read Volume on a Pipetman

Play Video
Almost done...

Centrifuge Vacuum Problems

Play Video
Almost done...

Tissue Prep for LCMS - Pt.8: Final Desalting

Play Video
Almost done...

Centrifuge problem, speed and tach errors

Play Video

Newsletter

Subscribe with thousands of Scientists

Recent Blog Articles

Using Your PhD Outside of Research

May 6, 2013

Dear Dora, Are there certain career paths outside of research where the PhD dramatically helps your career advancement? I’m a third-year grad student planning on leaving the bench (to do what, I’m not sure) but I feel like I’m half way there so if getting the letters is important I could tough it out. —MM, …

Read Full Article

The Broken Graduate Education Experience

April 11, 2013

The life of a scientist can be very hard. Some of us find ourselves fighting through graduate school and postdoctoral fellowships, battling for jobs in a saturated job market, and then bootstrapping our way through the progression of our career trajectory. Regardless of that, obtaining a PhD is extraordinarily rewarding and impactful to society! Let’s …

Read Full Article

The Conferencation: Adding Personal Time to a Scientific Meeting

March 29, 2013

Dear Dora, Any tips on how to tack a vacation on to a conference without my PI getting angry? I’m traveling to a conference in a great location and I want to stay an extra week after the conference (which itself is a week long), but I’m really nervous to tell the boss.  -RJ, graduate …

Read Full Article

  • Who is BenchFly?

    Our Mission
    Who's Involved
    Advisors
    Privacy
    Terms & Conditions

  • Services

    BenchFly Reactor
    Full Production
    Video Hosting

    Strategy & Consulting

  • Video Protocols

    Biology
    Chemistry
    DNA/RNA
    Protein
  • Sign Up Today

    Sign Up Today

    Twitter
    Facebook
    Contact Us
Copyright © 2013 BenchFly.   All Rights Reserved.