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Tissue Prep for LCMS - Pt.4: Desalting

By Ayers Institute 2975 Views
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In this step, we remove the salts from the digested peptides before moving on to isoelectric focusing.

Acetonitrile, HPLC Grade

Supplier: Fisher Scientific
Catalog Number: A998-1

Nunc deep well plates, 2mL sterile

Supplier: Nunc
Catalog Number: 278743

Oasis HLB 96-well plate uElution Plate 30um

Supplier: Waters
Catalog Number: 1860001828BA

Oasis HLB 96-well plate, 30um (10mg)

Supplier: Waters
Catalog Number: 186000128

Oasis HLB 96-well, 30um (5mg)

Supplier: Waters
Catalog Number: 186000309

SEP-Pak vac 1cc, 100mg, C18

Supplier: Waters
Catalog Number: 023590

Water, HPLC Grade

Supplier: VWR
Catalog Number: JT9823-1

Adobe PDF File Text Version of the Protocol

See the attached file for the text version of the protocol and check out the other videos in the Tissue Prep for LCMS series.

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