BenchFly
  • Join
  • Login
  • What is BenchFly?
  • Video Protocols
    • Biological Techniques
    • Chemical Techniques
    • DNA/RNA-related
    • Instruments and Equipment
    • Other
    • Protein-related
    • Proteomics
    • Useful Resources
    • Browse All
  • Productions
  • Blog
  • About

Tissue Prep for LCMS - Pt.4: Desalting

By Ayers Institute 2437 Views
Tweet

Add Your Own

Sign-up for BenchFly Free Today to Share Your Science Video Protocols

Sponsor

  • Summary
  • Products
  • Attachments
  • Notes

In this step, we remove the salts from the digested peptides before moving on to isoelectric focusing.

Acetonitrile, HPLC Grade

Supplier: Fisher Scientific
Catalog Number: A998-1

Nunc deep well plates, 2mL sterile

Supplier: Nunc
Catalog Number: 278743

Oasis HLB 96-well plate uElution Plate 30um

Supplier: Waters
Catalog Number: 1860001828BA

Oasis HLB 96-well plate, 30um (10mg)

Supplier: Waters
Catalog Number: 186000128

Oasis HLB 96-well, 30um (5mg)

Supplier: Waters
Catalog Number: 186000309

SEP-Pak vac 1cc, 100mg, C18

Supplier: Waters
Catalog Number: 023590

Water, HPLC Grade

Supplier: VWR
Catalog Number: JT9823-1

Adobe PDF File Text Version of the Protocol

See the attached file for the text version of the protocol and check out the other videos in the Tissue Prep for LCMS series.

Comments

Popular video protocols!

Using an autoclave

Using an autoclave

Play Video
An easy silica gel dispenser

An easy silica gel dispenser

Play Video
Pouring Acrylamide Gels for SDS-PAGE

Pouring Acrylamide Gels for SDS-PAGE

Play Video
Making Capillary Tubes

Making Capillary Tubes

Play Video
How to mail DNA

How to mail DNA

Play Video
Comparing Capillary Cutters for LCMS

Comparing Capillary Cutters for LCMS

Play Video

Sponsor

Become a BenchFly Sponsor

Newsletter

Subscribe with thousands of Scientists

Recent Blog Articles

From Academic Bench Chemist to Freelance Science Writer

February 22, 2012

Given that finely-honed writing skills are an essential component of successful research, it should come as no surprise that many graduate students and postdocs gravitate toward science journalism as a career option. What is surprising, however, is the lack of guidance we’re given in pursuit of this goal. To see how one scientist made the …

Read Full Article

The Forgotten Element of Graduate School: Coursework

February 20, 2012

A member of my dissertation committee once remarked “Grades will keep you in graduate school but research will get you out.” The implication was that coursework is a necessary evil on the road to a Ph.D.  Indeed, most of the discussions on BenchFly focus on research projects, research advisors, scoring the big paper or passing …

Read Full Article

A Quick Makeover: From ‘Lab Drab’ to ‘Ab Fab’ in 10 Minutes

February 17, 2012

By the end of the day I feel like I’m covered in “lab”. So when my friends want to go out after work I really feel like I need to go home to regroup, but home is too far away- not practical.  Is there anything I can do in the dingy bathroom at lab to …

Read Full Article

  • BenchFly

    Welcome to BenchFly
    What is BenchFly?
    Our Mission and Advisors
    Privacy
    Terms and Conditions
    Contact Us

  • Get Started

    Read Blogs
    Submit a Blog
    Upload a Video
    Sign-Up for a Free Account

  • Video Protocols

    Browse All
    Biological Techniques
    Chemical Techniques
    DNA/RNA-related
    Instruments
    Other
    Protein-related
    Proteomics
    Useful Resources

  • Stay Connected

    Email Newsletter
    RSS
    Twitter
    Facebook

Copyright © 2012 BenchFly.   All Rights Reserved.