BenchFly
  • Join
  • Login
  • What is BenchFly?
  • Video Protocols
    • Biological Techniques
    • Chemical Techniques
    • DNA/RNA-related
    • Instruments and Equipment
    • Other
    • Protein-related
    • Proteomics
    • Useful Resources
    • Browse All
  • Productions
  • Blog
  • About

Tissue Prep for LCMS - Pt.5: ZOOM Isoelectric Focusing (IEF)

By Ayers Institute 3196 Views
Tweet

Add Your Own

Sign-up for BenchFly Free Today to Share Your Science Video Protocols

Sponsor

  • Summary
  • Products
  • Attachments
  • Notes

This the standard protocol we use for separating digested peptides by isoelectric focusing prior to LCMS analysis.

Acetonitrile, HPLC Grade

Supplier: Fisher Scientific
Catalog Number: A998-1

Falcon 96-well Microplates, clear, flat-bottom wtih lid

Supplier: BD Biosciences
Catalog Number: 351172

Nunc deep well plates, 2mL sterile

Supplier: Nunc
Catalog Number: 278743

Urea

Supplier: Fisher Scientific
Catalog Number: BP169-500

Water, HPLC Grade

Supplier: VWR
Catalog Number: JT9823-1

ZOOM Custom Order pH Strips (pH 3.5-4.7)

Supplier: Invitrogen
Catalog Number: Custom order

ZOOM Dual Power (100-120 VAC 47-60Hz)

Supplier: Invitrogen
Catalog Number: ZM1001

ZOOM IPGRunner Cassettes

Supplier: Invitrogen
Catalog Number: ZM0003

ZOOM IPGRunner Mini-Cell

Supplier: Invitrogen
Catalog Number: ZM0001

Adobe PDF File Text Version of the Protocol

See the attached file for the text version of the protocol and check out the other videos in the Tissue Prep for LCMS series.

Comments

Popular video protocols!

How to reduce bumping on a rotovap

How to reduce bumping on a rotovap

Play Video
HighWire Literature Search

HighWire Literature Search

Play Video
Creating an aspiration apparatus

Creating an aspiration apparatus

Play Video
How to remove static from a scale

How to remove static from a scale

Play Video
The Smart Way to Your Ph.D.

The Smart Way to Your Ph.D.

Play Video
Opening a locked J2-21

Opening a locked J2-21

Play Video

Sponsor

Become a BenchFly Sponsor

Newsletter

Subscribe with thousands of Scientists

Recent Blog Articles

Jean Expression

May 18, 2012

We all know that gene regulation is an important aspect for the function of the gene in a cell.   Well, I am willing to argue that the regulation is equally important in the facilitation your own personal jean expression.  How do you want to feel when you don your denim?  Since you’re reading this article, …

Read Full Article

This is Not an Article About Running Viruses…

May 16, 2012

…but when I saw the headline “scientists generate electricity from viruses” this is where my mind went: Just hook that little guy up to a generator, and boom! Electricity! Sadly that’s not really how these scientists are making electricity from viruses. I mean, if I really think about it, there are several flaws to my …

Read Full Article

Who’s Really Pursuing the Alternative Career in Science?

May 15, 2012

These days it’s hard to escape the “alternative” label – alternative energy, alternative lifestyles, alternative medicine, alternative music, and of course, alternative careers. Prefacing a term with “alternative” implies those who find themselves in the “alternative” category have somehow strayed from the “normal” or “accepted” track. Nowhere is this stigma felt more than in science, …

Read Full Article

  • BenchFly

    Welcome to BenchFly
    What is BenchFly?
    Our Mission and Advisors
    BenchFly Around the World
    Privacy
    Terms and Conditions
    Contact Us

  • Get Started

    Read Blogs
    Upload a Video
    Sign-Up for a Free Account

  • Video Protocols

    Browse All
    Biological Techniques
    Chemical Techniques
    DNA/RNA-related
    Instruments
    Other
    Protein-related
    Proteomics
    Useful Resources

  • Stay Connected

    Email Newsletter
    RSS
    Twitter
    Facebook

Copyright © 2012 BenchFly.   All Rights Reserved.