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Pouring Acrylamide Gels for SDS-PAGE

By Alan M. 6095 Views
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Here's how to pour acrylamide gels using a BioRad system. Although the plates may vary with other systems, the technique and recipes will still be applicable.

30% acrylamide/bis solution 19:1

Supplier: BioRad
Catalog Number: 161-0154

Ammonium persulfate (APS)

Supplier: SigmaAldrich
Catalog Number: A3678

N,N,N',N'-Tetramethylethylenediamine (TEMED)

Supplier: SigmaAldrich
Catalog Number: T9281

Sodium dodecyl sulfate (SDS; Lauryl sulfate sodium salt)

Supplier: SigmaAldrich
Catalog Number: L3771

Adobe PDF File Text Version of the Protocol

See attached file for buffer recipes.

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