Video Protocols > How to make a bacterial spreader
How to make a bacterial spreader
By Alan M. 1823 Views
Beginner
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Summary
Making your own bacterial spreader is fast and easy as we demonstrate in this video - No words necessary...it's that easy!
Notes
Music: Making me nervous, by Brad Sucks.
Products Used in This Protocol
100x20 cell culture dish
Supplier: BD Biosciences
Catalog Number: 353003
Disposable Pasteur Pipets
Supplier: VWR
Catalog Number: 14672-380
Standard Bunson Burner with flame stabilizer
Supplier: Humboldt
Catalog Number: H-5870
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Comments (9)
"I actually do the final flame so that the spreader is curved on both sides - makes it easier to use. If you don't do it, sometimes the straight end catches on the agar and can break the spreader."
- Alan M., Aug 6 @ 8:59 pm
"Did you have to make sure the hole in the end of the pipet got sealed up so that when you dip the spreader in ethanol and flame, the ethanol doesn' t go in the hole and explode back out?"
- Cdas, Aug 6 @ 8:29 pm
"Perfecto! Get asked this all the time. You nailed it!
betsykip"
- betsykip, Aug 6 @ 7:44 pm
"Hahaha- got a video for that?..."
- Alan M., Aug 6 @ 5:31 pm
"Very simple, but not quite as fun as making TLC spotters out of pasteur pipettes."
- PeterMadrid, Aug 6 @ 4:20 pm
"Love it! My attempts at making bacterial spreaders have never worked out like that. I'll give it a go.."
- joanneg, Aug 6 @ 12:10 pm
"FANTASTIC - that's my favorite video so far!"
- Kirem, Aug 6 @ 9:05 am
"Loved it--great music choice also.
Diane Felmet"
- Diane, Aug 6 @ 8:15 am
"The video is genius!!"
- Kathryn, Aug 6 @ 6:41 am