http://www.benchfly.com/video/189/microaggregate-isolation-protocol/ http://cdn-thumbs.viddler.com/thumbnail_2_12c5e6f6.jpg Microaggregate Isolation Protocol Introduction by Johan Six. This protocol allows you to physically separate microaggregates with macroaggregates (Mm) and free microaggregates (m). http://www.viddler.com/player/12c5e6f6/ 8020 2011-06-16 Soil Fractionation Organic Matter Carbon Cycling Carbon Stabilization Aggregates Microaggregate Isolation yes JenDen http://www.benchfly.com/video/183/soil-fractionation-protocol/ http://cdn-thumbs.viddler.com/thumbnail_2_8ab0430.jpg Soil Fractionation Protocol Introduction by Johan Six. This protocol allows you to physically isolate different aggregate size classes by wet sieving http://www.viddler.com/player/8ab0430/ 9730 2011-05-24 Soil Fractionation Soil Organic Matter Carbon cycling aggregates yes JenDen http://www.benchfly.com/video/177/life-cycle-of-a-hazardous-waste-bottle/ http://cdn-thumbs.viddler.com/thumbnail_2_fcacf1e2.jpg Life Cycle of a Hazardous Waste Bottle In this video we walk through the life cycle of a hazardous waste bottle to demonstrate the key steps at each stage. Proper management of a waste stream is essential to ensuring the waste is disposed of safely. http://www.viddler.com/player/fcacf1e2/ 16280 2011-04-11 hazardous waste waste bottle laboratory techniques waste stream saa maa satellite accumulation area main accumulation area yes ISN_MIT http://www.benchfly.com/video/175/your-pipette-and-you,-part-i/ http://cdn-thumbs.viddler.com/thumbnail_2_524a5240.jpg Your Pipette and You, Part I The micropipettor is one of the most used tools in the biochemist's and molecular biologist's toolbox. Learn to use it properly and your data will be so much better! http://www.viddler.com/player/524a5240/ 38940 2011-02-18 Pipetting Lab Techniques yes Teaster Baird Jr. http://www.benchfly.com/video/174/how-to-find-your-h-index/ http://cdn-thumbs.viddler.com/thumbnail_2_39c6edb7.jpg How to Find Your H-index In this short video, we show how to find your h-index, a metric that describes the scientific productivity and impact of a researcher. http://www.viddler.com/player/39c6edb7/ 18390 2010-10-20 h-index impact factor h index laboratory techniques journal publication citation citations hirsch yes Alan M. http://www.benchfly.com/video/173/openwetware-open-notebook/ http://cdn-thumbs.viddler.com/thumbnail_2_94e3bb85.jpg OpenWetWare Open Notebook Open Notebook Science is an up and coming aspect to working in the lab. Every lab has their own method of sharing research and we at the KochLab use OpenWetWare.org's notebook feature to make all of our protocols and data public. Here is how I organize and share my notes using all the web has to offer. http://www.viddler.com/player/94e3bb85/ 19330 2010-10-06 openwetware open notebook open notebook science kochlab, yes anthonysalvagno http://www.benchfly.com/video/172/how-to-use-a-fluorescence-microscope/ http://cdn-thumbs.viddler.com/thumbnail_2_2a1a099e.jpg How to Use a Fluorescence Microscope Here we demonstrate the basic features of a fluorescence microscope and how to use them. http://www.viddler.com/player/2a1a099e/ 43360 2010-08-09 scope fluorescent microscopy immunofluorescence immuno imaging laboratory techniques, yes Vanessa http://www.benchfly.com/video/171/how-to-use-a-light-microscope/ http://cdn-thumbs.viddler.com/thumbnail_2_bae50682_v4.jpg How to Use a Light Microscope This video demonstrates the basics of using a light microscope. http://www.viddler.com/player/bae50682/ 41980 2010-08-05 microscopy introduction intro laboratory techniques slide scope yes Vanessa http://www.benchfly.com/video/170/how-to-prepare-a-slide-for-the-light-microscope/ http://cdn-thumbs.viddler.com/thumbnail_2_31d1131b_v4.jpg How to Prepare a Slide for the Light Microscope Here's how to prepare a liquid sample for viewing under a light microscope. http://www.viddler.com/player/31d1131b/ 31510 2010-08-05 microscopy culture yeast bacteria laboratory techniques liquid yes Vanessa http://www.benchfly.com/video/169/how-to-chunk-worms-(c.elegans)/ http://cdn-thumbs.viddler.com/thumbnail_2_b4577c1d.jpg How to Chunk Worms (C.elegans) Chunking is the process of transferring worms (C.elegans) from one agar plate to another. It is one of the essential laboratory techniques in expanding healthy worms for experimentation or maintenance. Here we demonstrate the basic chunking protocol. http://www.viddler.com/player/b4577c1d/ 24400 2010-08-05 c elegans worm laboratory techniques microscope, yes Alice http://www.benchfly.com/video/168/visualizing-starved-worms-(c.elegans)-over-time/ http://cdn-thumbs.viddler.com/thumbnail_2_1e37b584_v4.jpg Visualizing Starved Worms (C.elegans) Over Time Once C.elegans deplete their bacterial food source and enter starvation mode, their appearance will change with length of starvation. Here we examine non-starved, 2-week starved and 6-week starved worms to compare what each sample should look like. http://www.viddler.com/player/1e37b584/ 20630 2010-08-05 c elegans worms starvation starve laboratory techniques microscope, yes Alice http://www.benchfly.com/video/167/preparing-a-two-sample-flow-cell/ http://cdn-thumbs.viddler.com/thumbnail_2_e158e68e_v2.jpg Preparing a Two Sample Flow Cell This video illustrates how to make a double sample flow cell, where 2 microfluidics experiments can be run side-by-side. We use this setup typically in our DNA tethering experiments. The setup is composed of a slide, a cover slip, and double-sided tape. http://www.viddler.com/player/e158e68e/ 27700 2010-06-19 cell flow kinesin optical tweezers microfluidics koch lab yes anthonysalvagno http://www.benchfly.com/video/166/preparing-a-flow-cell/ http://cdn-thumbs.viddler.com/thumbnail_2_202e02d8_v5.jpg Preparing a Flow Cell This video illustrates the preparation of a single channel flow cell. The cell is made up of a slide, a cover slip, and two pieces of double-sided tape. Liquids can be flowed into the chamber via capillary action. Excess liquied can be swept up with a kim wipe. http://www.viddler.com/player/202e02d8/ 20970 2010-06-19 kinesin koch lab microfluidics optical tweezers, yes anthonysalvagno http://www.benchfly.com/video/165/how-to-remove-bubbles-when-pouring-lb-agar-plates/ http://cdn-thumbs.viddler.com/thumbnail_2_55cb46b_v2.jpg How to Remove Bubbles When Pouring LB-Agar Plates Here's an easy way to get rid of any bubbles when pouring LB-agar plates. Bubbles should be avoided because they can make spreading bacteria and identifying of colonies difficult. http://www.viddler.com/player/55cb46b/ 34500 2010-06-09 lb agar dna colony laboratory techniques current protocols molecular biology biology biochemistry yes Alice http://www.benchfly.com/video/164/how-to-pour-lb-agar-plates/ http://cdn-thumbs.viddler.com/thumbnail_2_f2526e23_v2.jpg How to Pour LB-Agar Plates Pouring LB-agar plates is an essential laboratory technique for molecular biology. Here's an easy method for pouring them. http://www.viddler.com/player/f2526e23/ 46540 2010-06-08 dna agar luria plates colony laboratory techniques current protocols molecular biology biology biochemistry yes Alice http://www.benchfly.com/video/163/how-to-cut-tlc-plates/ http://cdn-thumbs.viddler.com/thumbnail_2_79045c90_v3.jpg How to Cut TLC Plates This video demonstrates how to cut glass-backed plates for thin layer chromatography. http://www.viddler.com/player/79045c90/ 24860 2010-05-23 glass tlc thin layer chromatography laboratory techniques science videos current protocols silica chemistry chemical biology yes Bruce M http://www.benchfly.com/video/161/how-to-cool-lb-agar-quickly/ http://cdn-thumbs.viddler.com/thumbnail_2_91f0a9b9.jpg How to Cool LB-Agar Quickly After autoclaving media, waiting for it to cool can take a long time so here's an easy way to bring the temp down quickly so you can pour your plates and get on with your day! http://www.viddler.com/player/91f0a9b9/ 22900 2010-05-13 laboratory techniques science videos current protocols bacteria plates biology tip biochemistry molecular biology, yes Alice http://www.benchfly.com/video/160/how-to-quickly-pour-a-small-number-of-lb-agar-plates/ http://cdn-thumbs.viddler.com/thumbnail_2_9427dea2.jpg How to Quickly Pour a Small Number of LB-Agar Plates Sometimes you just need a few LB plates, but making up the media, autoclaving, pouring and cooling can be a lengthy process. Here's a quick tip to pour a small number of plates quickly. http://www.viddler.com/player/9427dea2/ 19260 2010-05-13 laboratory techniques science videos current protocols LB plates bacteria biology biochemistry molecular biology tip cloning yes Alice http://www.benchfly.com/video/159/benchfly-public-service-announcement/ http://cdn-thumbs.viddler.com/thumbnail_2_84ab600d_v6.jpg BenchFly Public Service Announcement We've got a few things to say- and some dancing stick figures to help us out... http://www.viddler.com/player/84ab600d/ 13470 2010-05-12 laboratory techniques current protocols science videos stick figure, yes Alan M. http://www.benchfly.com/video/158/gel-red-vs-etbr/ http://cdn-thumbs.viddler.com/thumbnail_2_4d20d6db_v2.jpg Gel Red vs EtBr Comparing Gel-red DNA stain to EtBr. The Gel-red is in sample whereas the EtBr is in gel. All conditions are the same except for the DNA stains. I am using 1X TAE buffer and a VWR agarose. http://www.viddler.com/player/4d20d6db/ 112650 2010-05-04 Kirkwood Community College Greg Petersen Biotech electrophoresis Gel-red Ethidium Bromide DNA Ladder NEB Owl yes gkpeter http://www.benchfly.com/video/157/tip-for-selecting-capillary-tubing-to-plumb-lcms/ http://cdn-thumbs.viddler.com/thumbnail_2_c2e77879_v2.jpg Tip for Selecting Capillary Tubing to Plumb LCMS Plumbing an LCMS requires a significant amount of capillary tubing. When deciding on which spool of capillary tubing to use, here's a tip that may help minimize variability in runs. http://www.viddler.com/player/c2e77879/ 19400 2010-04-26 laboratory techniques current protocols mass spectrometer spectrometry proteomics chromatography yes Ayers Institute http://www.benchfly.com/video/156/estimating-volume-in-pipet-tips/ http://cdn-thumbs.viddler.com/thumbnail_2_545d9135_v2.jpg Estimating Volume in Pipet Tips Learning to recognize volume levels in pipet tips is an important instinct to develop. Here's a guide for estimating P20 volumes. http://www.viddler.com/player/545d9135/ 26140 2010-04-26 laboratory techniques science videos current protocols biology biochemistry micropipette micropipet pipetman molecular biology yes Alice http://www.benchfly.com/video/155/tip-for-working-with-small-volumes/ http://cdn-thumbs.viddler.com/thumbnail_2_d8a3d0d9_v2.jpg Tip for Working With Small Volumes When adding small volumes to reactions, it's easy to question whether the liquid was actually added or not. Here's a tip that will let you rest assured that everything was setup properly. http://www.viddler.com/player/d8a3d0d9/ 30270 2010-04-26 droplet laboratory techniques science videos current protocols biology molecular biology tip biochemistry pipetman pcr digest yes Alice http://www.benchfly.com/video/154/how-to-access-lcms-data-in-analyst-software/ http://cdn-thumbs.viddler.com/thumbnail_2_7276d184_v4.jpg How to Access LCMS Data in Analyst Software After running a sample on the LCMS, here's how to retrieve the spectrum and isolate desired peaks. http://www.viddler.com/player/7276d184/ 16810 2010-04-26 analyst lcms proteomics laboratory techniques science videos current protocols mass spec spectrometer yes Bogyo Lab http://www.benchfly.com/video/153/how-to-use-graduated-cylinders/ http://cdn-thumbs.viddler.com/thumbnail_2_b47f3c3e_v2.jpg How to Use Graduated Cylinders Here's a brief introduction on how to use graduated cylinders - including how to select the proper size and how to read volume accurately. http://www.viddler.com/player/b47f3c3e/ 24380 2010-04-26 graduated cylinders volume laboratory techniques science videos current protocols chemistry biology biochemistry measure yes Alice http://www.benchfly.com/video/152/how-to-read-volume-on-a-pipetman/ http://cdn-thumbs.viddler.com/thumbnail_2_f988773a_v2.jpg How to Read Volume on a Pipetman Reading pipetman will eventually become second nature, but when first starting out, it can be easy to get tripped up. Here's a quick tutorial on reading the volume in P20, P200 and P1000 pipetman. http://www.viddler.com/player/f988773a/ 29610 2010-04-26 P20 P200 P100 pipetman laboratory techniques science videos current protocols micropipette biology biochemistry molecular biology yes Alice http://www.benchfly.com/video/151/how-to-use-a-pipetman/ http://cdn-thumbs.viddler.com/thumbnail_2_41cbe53f_v2.jpg How to Use a Pipetman Pipetman are an essential part of biological research. Here's an introduction on how to use them properly. http://www.viddler.com/player/41cbe53f/ 35040 2010-04-26 pipetman pipetteman laboratory techniques science videos current protocols biology biochemistry introduction beginner molecular biology yes Alice http://www.benchfly.com/video/150/ph-meter-calibration/ http://cdn-thumbs.viddler.com/thumbnail_2_c7f5ce09_v4.jpg pH meter calibration Shows how to calibrate a pH meter using a 3-point calibration. http://www.viddler.com/player/c7f5ce09/ 29520 2010-04-02 Kirkwood biotechnology ph meter calibration electrode calomel standard yes gkpeter http://www.benchfly.com/video/139/using-an-autoclave/ http://cdn-thumbs.viddler.com/thumbnail_2_c6f59931_v4.jpg Using an autoclave Information on using an autoclave for typical molecular biology reagents and supplies. http://www.viddler.com/player/c6f59931/ 22720 2010-03-01 autoclave,kirkwood,sterile,safety,steam yes gkpeter http://www.benchfly.com/video/138/comparing-capillary-cutters-for-lcms/ http://cdn-thumbs.viddler.com/thumbnail_2_48640347_v12.jpg Comparing Capillary Cutters for LCMS These two different methods of cutting capillaries can result in major differences in the cleanliness of the cut. Using the wrong technique may affect the subsequent chromatography and analysis. http://www.viddler.com/player/48640347/ 29170 2010-02-18 mass spec proteomics diamond cutter laboratory techniques current protocols science videos spectrometry ceramic yes charlie http://www.benchfly.com/video/137/bottle-labeling-tape-trick/ http://cdn-thumbs.viddler.com/thumbnail_2_c0364902_v2.jpg Bottle Labeling Tape Trick This is a pretty common technique - but a gem. If you've ever spent more than 1 second removing tape from a bottle, try this. http://www.viddler.com/player/c0364902/ 35210 2010-02-18 label laboratory techniques science videos current protocols biology tip molecular biology yes Alan M. http://www.benchfly.com/video/134/how-to-setup-and-run-lcms-software/ http://cdn-thumbs.viddler.com/thumbnail_2_2dacb8bb.jpg How to Setup and Run LCMS Software You can use this technique to upload a single sample or an entire set on the LCMS. http://www.viddler.com/player/2dacb8bb/ 25500 2010-02-11 lc/ms mass spec spectrometry laboratory techniques current protocols science videos, yes Bogyo Lab http://www.benchfly.com/video/133/tissue-prep-for-lcms---pt.8:-final-desalting/ http://cdn-thumbs.viddler.com/thumbnail_2_b02aea5e_v2.jpg Tissue Prep for LCMS - Pt.8: Final Desalting In this protocol, the final step of tissue preparation for mass spectrometry, peptides are desalted and prepared for mass spec analysis. http://www.viddler.com/player/b02aea5e/ 24710 2010-02-11 lc/ms proteomics mass spec spectrometry ayers protein sample laboratory techniques current protocols science videos C18 sep pak proteomics mass spectrometer, yes Ayers Institute http://www.benchfly.com/video/132/tissue-prep-for-lcms---pt.7-extracting-peptides-from-zoom-strip/ http://cdn-thumbs.viddler.com/thumbnail_2_40f414d9_v2.jpg Tissue Prep for LCMS - Pt.7 Extracting Peptides from ZOOM Strip After cutting the isoelectric focusing strips, we recover the peptides by extracting them using this procedure. http://www.viddler.com/player/40f414d9/ 23980 2010-02-11 lc/ms proteomics mass spec spectrometry ayers protein sample laboratory techniques current protocols science videos extraction proteomics yes Ayers Institute http://www.benchfly.com/video/131/tissue-prep-for-lcms---pt.4:-desalting/ http://cdn-thumbs.viddler.com/thumbnail_2_e1d64c2_v2.jpg Tissue Prep for LCMS - Pt.4: Desalting In this step, we remove the salts from the digested peptides before moving on to isoelectric focusing. http://www.viddler.com/player/e1d64c2/ 24360 2010-02-11 lc/ms proteomics mass spec spectrometry ayers protein sample laboratory techniques current protocols science videos c18 sep pak, yes Ayers Institute http://www.benchfly.com/video/129/tissue-prep-for-lcms---pt.3:-alkylation/digestion/ http://cdn-thumbs.viddler.com/thumbnail_2_cf93747e_v2.jpg Tissue Prep for LCMS - Pt.3: Alkylation/Digestion In the third step of tissue preparation for mass spectrometry, we reduce, alkylate and digest the tissue sample. http://www.viddler.com/player/cf93747e/ 24980 2010-02-11 lc/ms proteomics mass spec spectrometry ayers protein sample laboratory techniques current protocols science videos iodoacetamide trypsin tryptic yes Ayers Institute http://www.benchfly.com/video/128/how-to-get-a-reaction-sample-without-removing-septum/ http://cdn-thumbs.viddler.com/thumbnail_2_537646a3.jpg How to Get a Reaction Sample without Removing Septum Monitoring reaction progress by TLC is pretty standard, but having to unclamp the flask, remove the septum, take a sample, replace the septum and re-clamp is a pain. Here I show you how to reduce it to "take a sample". Done. http://www.viddler.com/player/537646a3/ 25610 2010-01-31 tlc capillary "laboratory techniques" "science videos" "current protocols" Chemistry yes Jonathan H http://www.benchfly.com/video/127/how-to-prepare-a-sample-for-nmr/ http://cdn-thumbs.viddler.com/thumbnail_2_8b2366e9_v4.jpg How to Prepare a Sample for NMR Here's how to prepare a sample from your solid or oil for 13C or 1H NMR analysis. http://www.viddler.com/player/8b2366e9/ 29040 2010-01-30 nuclear magnetic resonance c13 proton carbon "laboratory techniques" "current protocols" "science videos" chemistry yes Don http://www.benchfly.com/video/126/streaking-bacteria-for-a-single-colony/ http://cdn-thumbs.viddler.com/thumbnail_2_a5c5cd89_v4.jpg Streaking Bacteria for a Single Colony When working with bacteria, it's important to use a single colony to be sure the bacteria all contain the same DNA. This video demonstrates how to streak out bacteria in order to isolate a single colony. http://www.viddler.com/player/a5c5cd89/ 63920 2010-01-30 bacterial culture "laboratory techniques" science videos current protocols biology cloning molecular biology biochemistry dna yes Alice http://www.benchfly.com/video/125/scientist-turned-comedian/ http://cdn-thumbs.viddler.com/thumbnail_2_d292697.jpg Scientist Turned Comedian Science comedy video by Tim Lee of PowerPointComedian.com http://www.viddler.com/player/d292697/ 34300 2010-01-26 Science Humor Athlete Tiger Woods Nuclear Fission Powerpoint yes Tim Lee http://www.benchfly.com/video/124/how-to-gas-parasites-in-culture/ http://cdn-thumbs.viddler.com/thumbnail_2_b9017112_v2.jpg How to Gas Parasites in Culture When working with plasmodium falciparum in culture, it's important to return them to a low oxygen environment before placing them back in the incubator. http://www.viddler.com/player/b9017112/ 23720 2010-01-25 plasmodium falciparum laboratory techniques science videos current protocols biology cell biology tissue culture cell culture, yes Bogyo Lab http://www.benchfly.com/video/123/how-to-smear-parasites-for-counting/ http://cdn-thumbs.viddler.com/thumbnail_2_9af2862e_v2.jpg How to Smear Parasites for Counting Here is our method for preparing a cells to quantify parasitemia in red blood cells. http://www.viddler.com/player/9af2862e/ 28260 2010-01-25 plasmodium falciparum microscope slide laboratory techniques science videos current protocols biology cell biology, yes Bogyo Lab http://www.benchfly.com/video/122/blat---a-powerful-genetic-search-tool/ http://cdn-thumbs.viddler.com/thumbnail_2_150d93f2_v3.jpg BLAT - A Powerful Genetic Search Tool Not to be confused with BLAST, BLAT is a great tool in it's own right- one of my favorites. Great way to do easy genome searches- find related genes, chromosome locations and much more! http://www.viddler.com/player/150d93f2/ 27780 2010-01-19 blast database laboratory techniques science videos current protocols bioinformatics computational software demo yes mike http://www.benchfly.com/video/121/performing-a-pubmed-lit-search/ http://cdn-thumbs.viddler.com/thumbnail_2_da488b29_v9.jpg Performing a PubMed Lit Search Using the modified search string described here, you can perform powerful PubMed searches with minimal work. http://www.viddler.com/player/da488b29/ 19140 2010-01-19 literature laboratory techniques science videos current protocols bioinformatics computational demo software yes mike http://www.benchfly.com/video/120/how-to-load-and-run-sds-page-gels/ http://cdn-thumbs.viddler.com/thumbnail_2_298e1521_v3.jpg How to load and run SDS-PAGE gels Performing an entire experiment only to have your samples splash into adjacent wells at the end is the worst. Luckily, proper loading technique can prevent that tragedy... http://www.viddler.com/player/298e1521/ 40590 2010-01-19 protein laboratory techniques current protocols science videos biochemistry acrylamide biology western blot electrophoresis yes Alan M. http://www.benchfly.com/video/119/preventing-leaks-when-pouring-plates-for-sds-page/ http://cdn-thumbs.viddler.com/thumbnail_2_d856b1b_v5.jpg Preventing leaks when pouring plates for SDS-PAGE Old plates can get chipped and cracked- here's an easy way to continue to use them without the leaks. http://www.viddler.com/player/d856b1b/ 34190 2010-01-19 gels protein laboratory techniques tip trick current protocols science videos biochemistry biology western blot acrylamide electrophoresis yes Alan M. http://www.benchfly.com/video/118/tissue-prep-for-lcms---pt.2:-bca-assay/ http://cdn-thumbs.viddler.com/thumbnail_2_4e94e37e_v2.jpg Tissue Prep for LCMS - Pt.2: BCA Assay In the second step of preparing a tissue sample for mass spec, we quantify the total protein amounts before moving on to digest it. http://www.viddler.com/player/4e94e37e/ 27920 2010-01-16 lc/ms proteomics mass spec spectrometry ayers protein sample laboratory techniques current protocols science videos protein quantification yes Ayers Institute http://www.benchfly.com/video/117/tissue-prep-for-lcms---pt.6:-cutting-zoom-strips/ http://cdn-thumbs.viddler.com/thumbnail_2_5800ae6e_v2.jpg Tissue Prep for LCMS - Pt.6: Cutting ZOOM Strips After separating peptides by isoelectric focusing, we cut the strips for further analysis, as shown here. http://www.viddler.com/player/5800ae6e/ 23160 2010-01-16 laboratory techniques science videos current protocols proteomics protein peptide separation Isoelectric Focusing IEF spectrometry mass spec yes Ayers Institute http://www.benchfly.com/video/116/tissue-prep-for-lcms---pt.5:-zoom-isoelectric-focusing-(ief)/ http://cdn-thumbs.viddler.com/thumbnail_2_e14552d_v2.jpg Tissue Prep for LCMS - Pt.5: ZOOM Isoelectric Focusing (IEF) This the standard protocol we use for separating digested peptides by isoelectric focusing prior to LCMS analysis. http://www.viddler.com/player/e14552d/ 25840 2010-01-16 lc/ms proteomics mass spec spectrometry ayers protein sample laboratory techniques current protocols science videos IEF protein analysis yes Ayers Institute http://www.benchfly.com/video/115/tissue-prep-for-lcms---pt.1:-sonication/ http://cdn-thumbs.viddler.com/thumbnail_2_97aca5c8_v2.jpg Tissue Prep for LCMS - Pt.1: Sonication The first step in preparing tissue samples for LCMS analysis is to sonicate them as shown here. http://www.viddler.com/player/97aca5c8/ 31040 2010-01-16 lc/ms proteomics mass spec spectrometry ayers protein sample laboratory techniques current protocols science videos sonicate sonication, yes Ayers Institute http://www.benchfly.com/video/114/gene-searching-on-pubmed/ http://cdn-thumbs.viddler.com/thumbnail_2_2aa1c71f_v9.jpg Gene Searching on PubMed Here's some of the important information you can get from a basic gene search in PubMed. http://www.viddler.com/player/2aa1c71f/ 25890 2010-01-15 literature database dna laboratory techniques science videos current protocols bioinformatics computational demo software yes mike http://www.benchfly.com/video/113/serial-dilution/ http://cdn-thumbs.viddler.com/thumbnail_2_ad47f8f6_v2.jpg Serial Dilution Show how to do a serial dilution and the subsequent calculations to determine the final concentrations. http://www.viddler.com/player/ad47f8f6/ 29400 2010-01-15 Serial dilution kirkwood greg calculation pipette yes gkpeter http://www.benchfly.com/video/112/micropipette-calibration-and-analysis/ http://cdn-thumbs.viddler.com/thumbnail_2_d74d7561_v2.jpg Micropipette Calibration and analysis http://www.viddler.com/player/d74d7561/ 41000 2010-01-14 micro pipette kirkwood greg petersen calibration spreadsheet accuracy precision analytical balance yes gkpeter http://www.benchfly.com/video/111/dna-electrophoresis-sample-loading-examples/ http://cdn-thumbs.viddler.com/thumbnail_2_cac13fc6_v2.jpg DNA Electrophoresis sample loading examples Just a quick video showing how to load a DNA horizontal electrophoresis gel. Shows a close-up of the loading and some common mistakes when loading. http://www.viddler.com/player/cac13fc6/ 60740 2010-01-13 DNA electrophoresis sample buffer biotechnology kirkwood sample bubbles yes gkpeter http://www.benchfly.com/video/110/dna-horizontal-gel-electrophoresis/ http://cdn-thumbs.viddler.com/thumbnail_2_5aa56959_v2.jpg DNA Horizontal Gel Electrophoresis http://www.viddler.com/player/5aa56959/ 38810 2010-01-12 Electrophoresis DNA Biotechnology TAE agarose loading sample yes gkpeter http://www.benchfly.com/video/109/making-frits-for-lc/ms/ http://cdn-thumbs.viddler.com/thumbnail_2_7ca61fd6_v7.jpg Making Frits for LC/MS This is the technique we use to make frits for our LC/MS. http://www.viddler.com/player/7ca61fd6/ 26950 2010-01-12 lcms mass spec capillary laboratory techniques current protocols science videos spectrometry proteomics yes Ayers Institute http://www.benchfly.com/video/108/reusing-agarose-gels/ http://cdn-thumbs.viddler.com/thumbnail_2_6ca1a0b7.jpg Reusing agarose gels This is how I reuse agarose gels for my genotyping since I run large numbers of samples every few days - saving me time and money. http://www.viddler.com/player/6ca1a0b7/ 34980 2010-01-12 dna genotyping agarose gels laboratory techniques science videos current protocols biology molecular biology biochemistry pcr cloning yes rachel http://www.benchfly.com/video/107/highwire-literature-search/ http://cdn-thumbs.viddler.com/thumbnail_2_725b2245_v43.jpg HighWire Literature Search HighWire is a great database for performing lit searches. Here I show a couple of the important features on the site. http://www.viddler.com/player/725b2245/ 21640 2010-01-06 lit search journal journals current protocols laboratory techniques science videos bioinformatics computational demo software yes mike http://www.benchfly.com/video/106/pouring-acrylamide-gels-for-sds-page/ http://cdn-thumbs.viddler.com/thumbnail_2_ebef6869_v10.jpg Pouring Acrylamide Gels for SDS-PAGE Here's how to pour acrylamide gels using a BioRad system. Although the plates may vary with other systems, the technique and recipes will still be applicable. http://www.viddler.com/player/ebef6869/ 51320 2009-12-19 sds page electrophoresis protein laboratory techniques current protocols science videos temed aps gels western blot biochemistry biology ammonium biorad, yes Alan M. http://www.benchfly.com/video/105/a-tip-for-loading-the-ladder-in-sds-page/ http://cdn-thumbs.viddler.com/thumbnail_2_eab25eb5_v8.jpg A tip for loading the ladder in SDS-PAGE No need to dial the pipetman back and forth between the extreme volumes - here's an easy way to load the right amount of ladder without changing volume at all! http://www.viddler.com/player/eab25eb5/ 30480 2009-12-15 bio rad protein ladder protein prestained precision plus laboratory techniques science videos current protocols protein biochemistry biology acrylamide western blot electrophoresis yes Alan M. http://www.benchfly.com/video/104/how-to-bomb-load-capillary-tips-or-frits-for-lc/ms/ http://cdn-thumbs.viddler.com/thumbnail_2_58048ea_v35.jpg How to Bomb-load Capillary Tips or Frits for LC/MS This is how we prepare and bomb load capillary tips and frits for use in LC/MS. http://www.viddler.com/player/58048ea/ 29580 2009-12-15 lc-ms lcms laboratory techniques science videos current protocols proteomics, yes Ayers Institute http://www.benchfly.com/video/102/how-to-pull-a-capillary-tip-for-lc/ms/ http://cdn-thumbs.viddler.com/thumbnail_2_15dabc57_v6.jpg How to pull a capillary tip for LC/MS This is how we pull capillary tubes to make tips for use on our LC/MS. http://www.viddler.com/player/15dabc57/ 24190 2009-12-11 LC-MS LCMS laboratory techniques current protocols science videos mass spec spectrometer proteomics, yes Ayers Institute http://www.benchfly.com/video/101/ucsc-visigene-and-allen-brain-atlas/ http://cdn-thumbs.viddler.com/thumbnail_2_f2735d8_v5.jpg UCSC Visigene and Allen Brain Atlas These are two great databases for visualizing expression data in a range of different assays. I'll show you how to get started in this video. http://www.viddler.com/player/f2735d8/ 24320 2009-12-11 imaging bioinformatics brain laboratory techniques current protocols science videos bioinformatics computational demo software yes mike http://www.benchfly.com/video/100/web-of-knowledge-literature-search/ http://cdn-thumbs.viddler.com/thumbnail_2_aa007e61.jpg Web of Knowledge Literature Search Web of Knowledge is a great database for performing lit searches. In this video, I'll show you how to get started and why it's useful. http://www.viddler.com/player/aa007e61/ 25390 2009-12-11 lit search bioinformatics journal web search laboratory techniques science videos current protocols bioinformatics computational demo software yes mike http://www.benchfly.com/video/99/how-to-work-up-a-reaction/ http://cdn-thumbs.viddler.com/thumbnail_2_a2040b38_v5.jpg How to work-up a reaction Here's a basic protocol for working up a reaction. Obviously, I've done it a few times... http://www.viddler.com/player/a2040b38/ 29690 2009-12-04 laboratory techniques science videos current protocols extraction work up chemistry sep funnel yes Jonathan H http://www.benchfly.com/video/98/calculate-molarity-fast/ http://cdn-thumbs.viddler.com/thumbnail_2_96209198.jpg Calculate Molarity Fast http://www.viddler.com/player/96209198/ 84570 2009-11-20 You only need one number yes Larry http://www.benchfly.com/video/97/making-capillary-tubes/ http://cdn-thumbs.viddler.com/thumbnail_2_6bdc3663.jpg Making Capillary Tubes Pulling some capillary tubes at the uOttawa undergraduate lab. http://www.viddler.com/player/6bdc3663/ 90320 2009-11-17 Capillary tubes uOttawa Chemistry Glass Bunsen burner yes Connor L http://www.benchfly.com/video/96/how-to-pack-a-capillary-column/ http://cdn-thumbs.viddler.com/thumbnail_2_e4701770_v6.jpg How to pack a capillary column Here's a protocol to pack your own capillary columns for LCMS- much cheaper than ordering pre-packed columns. http://www.viddler.com/player/e4701770/ 29330 2009-11-06 lcms mass spec chromatography proteomics spectrometry, yes charlie http://www.benchfly.com/video/95/how-to-use-a-semi-dry-transfer-apparatus/ http://cdn-thumbs.viddler.com/thumbnail_2_9647d3c2_v4.jpg How to use a semi-dry transfer apparatus This is a quick way to set up a protein transfer to nitrocellulose using a semi-dry apparatus. http://www.viddler.com/player/9647d3c2/ 70690 2009-10-30 Bio-Rad Biorad bio rad western blot science videos laboratory techniques current protocols protein biochemistry nitrocellulose biology, yes Alan M. http://www.benchfly.com/video/94/cotton-trick-for-column-chromatography/ http://cdn-thumbs.viddler.com/thumbnail_2_f2c61b1f_v6.jpg Cotton trick for column chromatography No more messing around with a stick to get the cotton or glass wool positioned properly. Use this trick and you'll be done in 5 seconds. http://www.viddler.com/player/f2c61b1f/ 26450 2009-10-23 silica gel glass wool science videos current protocols laboratory techniques benchfly chemistry chemical biology yes Bruce M http://www.benchfly.com/video/93/science-magic/ http://cdn-thumbs.viddler.com/thumbnail_2_8328668d.jpg Science Magic Researcher demonstrates the magical properties of flash paper. http://www.viddler.com/player/8328668d/ 22620 2009-10-15 Science Magic yes Vandy Man http://www.benchfly.com/video/92/how-to-wetpack-a-silica-gel-column/ http://cdn-thumbs.viddler.com/thumbnail_2_36b54823_v3.jpg How to wetpack a silica gel column Everyone's got their own style to packing a silica gel column, but this is how I do it. Fast, easy and reliable- just what I'm looking for... http://www.viddler.com/player/36b54823/ 38670 2009-10-14 chromatography pack pour science videos current protocols laboratory techniques benchfly chemistry chemical biology yes Bruce M http://www.benchfly.com/video/91/pouring-and-running-an-agarose-gel/ http://cdn-thumbs.viddler.com/thumbnail_2_d0edb382_v27.jpg Pouring and running an agarose gel Performing DNA agarose gel electrophoresis is one of the most common molecular biology techniques. Here I show how to pour agar gels in two of the most common types of gel boxes. http://www.viddler.com/player/d0edb382/ 56760 2009-10-07 dna molecular biology agar biology biochemistry pcr cloning, yes rachel http://www.benchfly.com/video/88/diy-uv-developing-chamber/ http://cdn-thumbs.viddler.com/thumbnail_2_73ccfbae_v2.jpg DIY UV developing chamber Eliminate the accidental UV exposure from your baymate...we've got one of these developing chambers on every bench. Cheap and easy. http://www.viddler.com/player/73ccfbae/ 18990 2009-09-28 ultraviolet tlc plates science videos current protocols laboratory techniques benchfly chemistry chemical biology yes Bruce M http://www.benchfly.com/video/87/how-to-calibrate-pipettors/ http://cdn-thumbs.viddler.com/thumbnail_2_28113d64_v19.jpg How to Calibrate Pipettors Calibrating pipets takes only a few minutes and should be done once a year to make sure your experiments are accurate. http://www.viddler.com/player/28113d64/ 45260 2009-09-24 pipetman pipetteman science videos lab techniques current protocols yes ronen http://www.benchfly.com/video/86/cam-staining-tlc-plates/ http://cdn-thumbs.viddler.com/thumbnail_2_2bc2bbcc_v5.jpg CAM staining TLC plates Ceric Ammonium Molybdate (CAM) stain. Good for detecting polyhydroxylated compounds. http://www.viddler.com/player/2bc2bbcc/ 36470 2009-09-17 ceric ammonium molybdate thin layer chromatography yes Jonathan H http://www.benchfly.com/video/85/tip-for-loading-many-gel-samples-quickly/ http://cdn-thumbs.viddler.com/thumbnail_2_25196559.jpg Tip for loading many gel samples quickly I use this technique when genotyping and running gels on large numbers of samples. It makes adding the loading dye to all of them very easy. http://www.viddler.com/player/25196559/ 42340 2009-09-17 shortcut timesaver trick agarose dna genotyping biology molecular biology biochemistry pcr cloning agarose acrylamide yes rachel http://www.benchfly.com/video/84/my-favorite-eppi-tubes/ http://cdn-thumbs.viddler.com/thumbnail_2_9bf15b85_v2.jpg My favorite eppi tubes Here are the eppi tubes I love and loath... Picking the wrong eppis is the worst- who knew such a small thing could cause such big trouble... http://www.viddler.com/player/9bf15b85/ 26520 2009-09-17 eppis review favorite biology molecular biology biochemistry, yes rachel http://www.benchfly.com/video/83/pcr-tube-strip-labeling-tip/ http://cdn-thumbs.viddler.com/thumbnail_2_2a6db880_v6.jpg PCR tube strip labeling tip There's not much room to write on small pcr tubes, so here's a time-saver that makes it much easier. http://www.viddler.com/player/2a6db880/ 41240 2009-09-17 tip trick shortcut strip tubes strips biology molecular biology biochemistry cloning dna yes rachel http://www.benchfly.com/video/82/keeping-the-rotovap-dry/ http://cdn-thumbs.viddler.com/thumbnail_2_4626e3f9.jpg Keeping the rotovap dry This easy fix prevents the huge puddle from forming under the cold finger of the rotovap. http://www.viddler.com/player/4626e3f9/ 18000 2009-09-11 rotovap tip rotavap rotary evaporator science videos current protocols laboratory techniques benchfly chemistry chemical biology yes Bruce M http://www.benchfly.com/video/81/the-smart-way-to-your-ph.d./ http://cdn-thumbs.viddler.com/thumbnail_2_96a71e30.jpg The Smart Way to Your Ph.D. Dora gives an overview of "The Smart Way to Your Ph.D.:200 Secrets from 100 Graduates" Find out more at www.yourphd.com. http://www.viddler.com/player/96a71e30/ 30440 2009-09-10 graduate school application resource guide,guidebook doctorate dissertation thesis rsi tendonitis adviser communication job search yes Dora http://www.benchfly.com/video/80/how-to-reduce-bumping-on-a-rotovap/ http://cdn-thumbs.viddler.com/thumbnail_2_82b19a89_v4.jpg How to reduce bumping on a rotovap Here's how to reduce bumping and minimize the damage in the event your sample still bumps while on the rotovap. http://www.viddler.com/player/82b19a89/ 29140 2009-09-09 rotovap rotavap bump guard science videos current protocols laboratory techniques benchfly chemistry chemical biology rotary evaporator yes Alan M. http://www.benchfly.com/video/79/recycled-ice-scoops/ http://cdn-thumbs.viddler.com/thumbnail_2_eb8b4aaa_v6.jpg Recycled ice scoops Here's an easy way to make recycled ice scoops out bottles that might otherwise have been thrown away. http://www.viddler.com/player/eb8b4aaa/ 23560 2009-09-03 recycle green scooper dry ice ice reuse science videos current protocols laboratory techniques benchfly chemistry chemical biology yes Bruce M http://www.benchfly.com/video/78/how-to-clean-a-rotovap/ http://cdn-thumbs.viddler.com/thumbnail_2_e705244b_v4.jpg How to clean a rotovap I do this about once a week to keep our rotovap clean. Great way to remove debris and condensation. http://www.viddler.com/player/e705244b/ 28630 2009-09-01 tip rotary evaporator acetone bath, yes Jonathan H http://www.benchfly.com/video/77/an-easy-silica-gel-dispenser/ http://cdn-thumbs.viddler.com/thumbnail_2_c5affe84.jpg An easy silica gel dispenser This silica gel dispenser is a great way to eliminate the carcinogenic cloud that often forms when taking silica gel from a bottle or drum. http://www.viddler.com/player/c5affe84/ 38350 2009-08-27 silica gel bottle sio2 tip cloud science videos current protocols laboratory techniques benchfly chemistry chemical biology yes Bruce M http://www.benchfly.com/video/75/extraction-of-limonene-using-liquid-carbon-dioxide/ http://cdn-thumbs.viddler.com/thumbnail_2_208384f7.jpg Extraction Of Limonene Using Liquid Carbon Dioxide We demonstrate using liquid CO2 to extract limonene from orange peel in a more environmentally friendly way than the traditional extraction using chlorinated hydrocarbons or steam distillation. http://www.viddler.com/player/208384f7/ 50710 2009-08-25 limonene extraction carbon dioxide green chemistry alternative yes lowlevelpanic999 http://www.benchfly.com/video/74/microwave-ball-lightning/ http://cdn-thumbs.viddler.com/thumbnail_2_b95f1302.jpg Microwave Ball Lightning We put some melted wax and burning matches in a microwave so you don't have to. http://www.viddler.com/player/b95f1302/ 26120 2009-08-24 southampton chemistry ball lightning plasma microwave yes lowlevelpanic999 http://www.benchfly.com/video/73/sirna-transfection-tip/ http://cdn-thumbs.viddler.com/thumbnail_2_4410036a.jpg siRNA transfection tip This is now my standard protocol for siRNA transfection. I found it increases the efficiency of knockdown, which can really improve cell-based experiments. http://www.viddler.com/player/4410036a/ 31820 2009-08-17 sirna trick laboratory techniques current protocols biology biochemistry chemical biology rna molecular biology yes Aaron J http://www.benchfly.com/video/72/how-to-leave-a-comment/ http://cdn-thumbs.viddler.com/thumbnail_2_3b392bbe_v12.jpg How to leave a comment This is the easiest way to leave a comment on BenchFly. Set it up once in under a minute and you won't have to login ever again! http://www.viddler.com/player/3b392bbe/ 26220 2009-08-17 intensedebate intense debate demo screenshot science videos current protocols laboratory techniques benchfly yes Alan M. http://www.benchfly.com/video/67/friday-knuckle-tatts/ http://cdn-thumbs.viddler.com/thumbnail_2_f71eee92_v3.jpg Friday Knuckle Tatts http://www.viddler.com/player/f71eee92/ 22100 2009-08-14 knuckle tatts friday video have a weekend yes Alan M. http://www.benchfly.com/video/66/how-to-calculate-an-rf-value/ http://cdn-thumbs.viddler.com/thumbnail_2_b40773ad_v3.jpg How to calculate an Rf value Calculating an Rf value is essential for communicating where the compound of interest runs in a given solvent system. http://www.viddler.com/player/b40773ad/ 107810 2009-08-10 tlc thin layer chromatography solvent front science videos current protocols laboratory techniques benchfly chemistry chemical biology silica, yes Alan M. http://www.benchfly.com/video/65/how-to-make-a-bacterial-spreader/ http://cdn-thumbs.viddler.com/thumbnail_2_3c311bc8_v2.jpg How to make a bacterial spreader Making your own bacterial spreader is fast and easy as we demonstrate in this video - No words necessary...it's that easy! http://www.viddler.com/player/3c311bc8/ 45850 2009-08-05 pasteur pipet glass pipet pipette spreader transformation science videos current protocols laboratory techniques benchfly biology biochemistry molecular biology bacteria yes Alan M. http://www.benchfly.com/video/61/make-your-own-rapid-ligation-kit/ http://cdn-thumbs.viddler.com/thumbnail_2_bd9d273f_v3.jpg Make your own Rapid Ligation Kit Save lots of time and money when cloning by making your own rapid ligation kit as I demonstrate here. http://www.viddler.com/player/bd9d273f/ 45600 2009-07-12 ligase rapid ligase 5 minute ligation fast ligation time-saving dna cloning molecular biology biology biochemistry laboratory techniques current protocols yes Sven L http://www.benchfly.com/video/60/how-do-you-say-hoechst?/ http://cdn-thumbs.viddler.com/thumbnail_2_3a600702_v2.jpg How do you say Hoechst? Trying to pronounce this nuclear stain can get embarrassing. Don't worry - it's not as hard as it looks... http://www.viddler.com/player/3a600702/ 70980 2009-07-12 hoechst dapi nuclear stain pronounce science videos current protocols laboratory techniques benchfly cell biology biology biochemistry imaging yes Alan M. http://www.benchfly.com/video/59/how-to-make-an-old-fashioned/ http://cdn-thumbs.viddler.com/thumbnail_2_bb9a7ae4_v2.jpg How to make an old-fashioned After a hard day in the lab, treat yourself to one of these! http://www.viddler.com/player/bb9a7ae4/ 35400 2009-07-12 drink whiskey relax benchlife yes Steve M http://www.benchfly.com/video/58/how-to-keep-a-clean-waterbath/ http://cdn-thumbs.viddler.com/thumbnail_2_5658f25_v2.jpg How to keep a clean waterbath A clean water bath is an important step in preventing bacterial and fungal infections. Watch this to keep your water bath from becoming a slime bath. http://www.viddler.com/player/5658f25/ 44080 2009-07-12 algicide water bath contamination fungus bacteria science videos current protocols laboratory techniques benchfly cell culture biology cell biology biochemistry chemistry instrument equipment yes Alan M. http://www.benchfly.com/video/57/how-to-perform-colony-pcr/ http://cdn-thumbs.viddler.com/thumbnail_2_99ce05f2_v2.jpg How to perform colony PCR Here's an easy way to rapidly screen ligation reactions for positive clones - this tip will change your molecular biology life. Allart Stoop showed me how to perform colony pcr, now I'm showing you. http://www.viddler.com/player/99ce05f2/ 126340 2009-07-11 colony bacteria bacterial PCR ligation screening science videos current protocols laboratory techniques benchfly molecular biology biology biochemistry dna cloning yes Alan M. http://www.benchfly.com/video/56/how-to-pull-capillary-tubes/ http://cdn-thumbs.viddler.com/thumbnail_2_76a3bce1_v2.jpg How to pull capillary tubes Making TLC spotters is fun and easy!! Well, at least easy... Here's a quick way to pull your own. http://www.viddler.com/player/76a3bce1/ 23360 2009-07-11 tlc thin layer chromatography pull tubes capillary spotter spotters science videos current protocols laboratory techniques benchfly chemistry pipet pipette chemical biology yes Alan M. http://www.benchfly.com/video/55/how-to-remove-static-from-a-scale/ http://cdn-thumbs.viddler.com/thumbnail_2_c767d9d0_v2.jpg How to remove static from a scale Scale acting like it has a mind of it's own? It might be static - try this quick fix to tame the beast. http://www.viddler.com/player/c767d9d0/ 51710 2009-07-11 static gun static scale won't tare tear troubleshooting science videos current protocols laboratory techniques benchfly chemistry biology instrument weight yes Alan M. http://www.benchfly.com/video/54/how-to-weigh-small-amounts/ http://cdn-thumbs.viddler.com/thumbnail_2_a7848591_v2.jpg How to weigh small amounts Using the right approach to weighing solids can dramatically improve the accuracy of your readings. http://www.viddler.com/player/a7848591/ 36920 2009-07-11 weigh paper scale science videos current protocols laboratory techniques benchfly chemistry biology biochemistry yes Alan M. http://www.benchfly.com/video/53/calculating-how-to-split-cells/ http://cdn-thumbs.viddler.com/thumbnail_2_e046bc9c_v2.jpg Calculating how to split cells Planning the split is often way harder than actually performing the split. Keep it simple by focusing on this one factor. http://www.viddler.com/player/e046bc9c/ 31930 2009-07-10 confluence cell density split surface area splitting science videos current protocols laboratory techniques benchfly biology biochemistry cell culture tissue culture culture cell biology yes Alan M. http://www.benchfly.com/video/52/sirna-transfection/ http://cdn-thumbs.viddler.com/thumbnail_2_e1d98e25_v4.jpg siRNA Transfection This is an effective technique for siRNA transfections that I use for mammalian cells. http://www.viddler.com/player/e1d98e25/ 36360 2009-07-10 sirna mammalian cells optimem lipofectamine biology biochemistry chemical biology rna laboratory techniques current protocols yes Aaron J http://www.benchfly.com/video/51/polypropylene-vs.-polystyrene/ http://cdn-thumbs.viddler.com/thumbnail_2_ff0b581b_v1.jpg Polypropylene vs. Polystyrene Here's how to distinguish polypropylene from polystyrene. Choose wisely... the wrong tubes will screw you. http://www.viddler.com/player/ff0b581b/ 69670 2009-07-10 conical bottom tubes centrifuge tubes maxiprep falcon biology molecular biology biochemistry tube selection, yes rachel http://www.benchfly.com/video/50/opening-a-locked-ultracentrifuge/ http://cdn-thumbs.viddler.com/thumbnail_2_4bb82228.jpg Opening a locked ultracentrifuge If the ultracentrifuge locks up, this is an easy way to open it up and save your samples. http://www.viddler.com/player/4bb82228/ 21370 2009-07-10 ultra centrifuge lid stuck release vacuum troubleshooting open door latch instrument laboratory techniques current protocols repair yes Tom D http://www.benchfly.com/video/49/opening-a-locked-eppendorf-centrifuge/ http://cdn-thumbs.viddler.com/thumbnail_2_87359cf.jpg Opening a locked Eppendorf centrifuge No need to worry if your tabletop centrifuge locks up - here's an easy way to open it right up. http://www.viddler.com/player/87359cf/ 22030 2009-07-10 tabletop centrifuge eppendorf 5415 5415C 5810 default the latch stuck lid tip freeze frozen instrument laboratory techniques current protocols repair yes Tom D http://www.benchfly.com/video/48/why-is-my-rotor-shaking?/ http://cdn-thumbs.viddler.com/thumbnail_2_4d916d1a.jpg Why is my rotor shaking? Some amount of rotor shaking may be normal, but it also may be part of a larger problem. Find out how to tell here. http://www.viddler.com/player/4d916d1a/ 20450 2009-07-10 rotor shake imbalance centrifuge laboratory techniques current protocols instrument repair yes Tom D http://www.benchfly.com/video/47/opening-a-locked-j2-21/ http://cdn-thumbs.viddler.com/thumbnail_2_c145da97.jpg Opening a locked J2-21 If the J2-21 freezes up, you can still get in even when it seems like youre locked out. http://www.viddler.com/player/c145da97/ 19550 2009-07-10 centrifuge defaulting the latch Beckman stuck lid troubleshooting broken laboratory techniques current protocols instrument tip locked frozen repair yes Tom D http://www.benchfly.com/video/46/opening-a-locked-sorvall-rc5c/ http://cdn-thumbs.viddler.com/thumbnail_2_1c49ce5e_v1.jpg Opening a locked Sorvall RC5C Here's an easy way to get in to a locked Sorvall RC5C without losing the samples inside. http://www.viddler.com/player/1c49ce5e/ 18380 2009-07-10 default the latch release stuck lid centrifuge instrument laboratory techniques current protocols lock locked frozen repair yes Tom D http://www.benchfly.com/video/45/opening-a-locked-rt6000/ http://cdn-thumbs.viddler.com/thumbnail_2_1917adc3.jpg Opening a locked RT6000 If your RT6000 is locked, don't panic- you can still get in. Here's how. http://www.viddler.com/player/1917adc3/ 16250 2009-07-10 default the latch RT7 sorvall stuck lid stuck centrifuge locked out laboratory techniques current protocols instrument repair yes Tom D http://www.benchfly.com/video/44/putting-a-centrifuge-rotor-on-properly/ http://cdn-thumbs.viddler.com/thumbnail_2_828f34c6_v2.jpg Putting a centrifuge rotor on properly Placing the rotor in the centrifuge properly is the first step to running the instrument safely. These tips can save you a lot of money in repairs! http://www.viddler.com/player/828f34c6/ 17750 2009-07-10 rotor pins imbalance damage centrifuge instrument laboratory techniques current protocols repair yes Tom D http://www.benchfly.com/video/43/centrifuge-problem,-speed-and-tach-errors/ http://cdn-thumbs.viddler.com/thumbnail_2_59d30ada.jpg Centrifuge problem, speed and tach errors If your instrument is showing a speed or tach error, there are a couple of things to check that may help fix the problem. http://www.viddler.com/player/59d30ada/ 19190 2009-07-10 tach error ultracentrifuge troubleshooting centrifuge broken repair instrument laboratory techniques current protocols fault yes Tom D http://www.benchfly.com/video/42/centrifuge-problem,-overtemp/ http://cdn-thumbs.viddler.com/thumbnail_2_fd9e6b50.jpg Centrifuge problem, overtemp If your centrifuge is over temp, follow these steps to see if you need to call for service. http://www.viddler.com/player/fd9e6b50/ 15190 2009-07-10 temperature error won't cool troubleshooting compressor not turning on instrument repair laboratory techniques current protocols fault yes Tom D http://www.benchfly.com/video/41/removing-a-stuck-centrifuge-rotor/ http://cdn-thumbs.viddler.com/thumbnail_2_e199117d.jpg Removing a stuck centrifuge rotor Here's how to help prevent getting a stuck centrifuge rotor and how to remove one should it happen. http://www.viddler.com/player/e199117d/ 24480 2009-07-10 preventing troubleshooting rotor extraction tool repair instrument laboratory techniques current protocols key frozen yes Tom D http://www.benchfly.com/video/40/swinging-bucket-rotor-care/ http://cdn-thumbs.viddler.com/thumbnail_2_845496f5_v2.jpg Swinging bucket rotor care Follow these steps to make sure the rotor is in good shape and avoid a costly malfunction. http://www.viddler.com/player/845496f5/ 17320 2009-07-10 bucket pins imbalance tabletop centrifuge repair instrument troubleshooting laboratory techniques current protocols yes Tom D http://www.benchfly.com/video/39/centrifuge-vacuum-problems/ http://cdn-thumbs.viddler.com/thumbnail_2_82c085da_v2.jpg Centrifuge vacuum problems If you're having trouble pulling a vacuum on your ultracentrifuge, check these parts to find the source of the problem. http://www.viddler.com/player/82c085da/ 17940 2009-07-10 troubleshooting ultracentrifuge o-ring oring won't pull vacuum broken repair laboratory techniques instrument current protocols yes Tom D http://www.benchfly.com/video/38/centrifuge-problems,-overspeed-disk/ http://cdn-thumbs.viddler.com/thumbnail_2_cf97a972.jpg Centrifuge problems, overspeed disk Be sure the overspeed disk is present on your ultracentrifuge rotors to ensure proper function. Without it there may be serious problems. http://www.viddler.com/player/cf97a972/ 21840 2009-07-10 troubleshooting centrifuge ultracentrifuge overspeed disk run won't start rotor won't spin centrifuge broken laboratory techniques instrument repair current protocols yes Bill M http://www.benchfly.com/video/37/centrifuge-imbalance-errors/ http://cdn-thumbs.viddler.com/thumbnail_2_bcb6a7ff.jpg Centrifuge imbalance errors If you're getting an imbalanced rotor, take a look at these possible sources in order to fix the problem. http://www.viddler.com/player/bcb6a7ff/ 20160 2009-07-10 imbalance problem troubleshooting L8-70M centrifuge error instrument laboratory techniques current protocols repair yes Tom D http://www.benchfly.com/video/36/centrifuge-cooling-problems/ http://cdn-thumbs.viddler.com/thumbnail_2_366319ca.jpg Centrifuge cooling problems Here are a couple of quick things to check when troubleshooting why your centrifuge isn't cooling properly. http://www.viddler.com/player/366319ca/ 19440 2009-07-10 L8-70M beckman J2-21 cooling problem cetrifuge won't cool troubleshooting fault repair laboratory techniques instrument current protocols yes Tom D http://www.benchfly.com/video/35/common-centrifuge-problem,-arc-button/ http://cdn-thumbs.viddler.com/thumbnail_2_b72d62b7_v9.jpg Common centrifuge problem, ARC button Centrifuge not starting? If you're having problems, check this simple fix first. http://www.viddler.com/player/b72d62b7/ 20050 2009-07-10 ARC automatic rate control centrifuge broken centrifuge won't start not starting broken RC5C troubleshooting sorvall laboratory techniques current protocols instrument repair yes Tom D http://www.benchfly.com/video/34/western-blot-transfer,-wet-apparatus/ http://cdn-thumbs.viddler.com/thumbnail_2_926ba3fd_v2.jpg Western blot transfer, wet apparatus Wet protein transfers to nitrocellulose are easy as long as you pay attention to a few key steps as demonstrated here. http://www.viddler.com/player/926ba3fd/ 71990 2009-07-09 western blot transfer nitrocellulose wet biorad transfer biology biochemistry laboratory techniques current protocols protein yes Brenda C http://www.benchfly.com/video/33/working-with-sterile-technique/ http://cdn-thumbs.viddler.com/thumbnail_2_424aa4d1_v2.jpg Working with sterile technique Good sterile technique will save you countless hours of contamination damage control- get started here. http://www.viddler.com/player/424aa4d1/ 35690 2009-07-09 sterile technique working in hood cell culture tissue culture beginner proper technique science videos current protocols laboratory techniques benchfly culture cells yes Alan M. http://www.benchfly.com/video/32/changing-tanks-on-a-gas-manifold/ http://cdn-thumbs.viddler.com/thumbnail_2_848d81d_v3.jpg Changing tanks on a gas manifold High pressure requires high attention... Here's a basic method for replacing your gas tanks. http://www.viddler.com/player/848d81d/ 21060 2009-07-09 co2 tanks changing gas tank change tank manifold gas science videos current protocols laboratory techniques benchfly biology biochemistry chemistry tank, yes Alan M. http://www.benchfly.com/video/31/timesaving-miniprep-tips/ http://cdn-thumbs.viddler.com/thumbnail_2_7dfc9bb0_v2.jpg Timesaving miniprep tips Purifying DNA by minpreps can be painfully boring. Implement these tips to get you through it faster. http://www.viddler.com/player/7dfc9bb0/ 35460 2009-07-09 miniprep eppi rack vacuum manifold qiagen science videos current protocols laboratory techniques benchfly biochemistry biology molecular biology dna bacteria plasmid, yes Alan M. http://www.benchfly.com/video/30/how-to-mail-dna/ http://cdn-thumbs.viddler.com/thumbnail_2_8dbea672_v2.jpg How to mail DNA No more styrofoam box hunts or FedEx account number searches. There's an easier way to mail DNA... http://www.viddler.com/player/8dbea672/ 45670 2009-07-09 mailing dna ship dna whatman paper mail dna science videos current protocols laboratory techniques benchfly biology biochemistry, yes Alan M. http://www.benchfly.com/video/29/pcr-buffer-review-(buffer-d,-epicentre)/ http://cdn-thumbs.viddler.com/thumbnail_2_7964b3bb_v2.jpg PCR Buffer Review (Buffer D, Epicentre) This PCR buffer never let me down and is one of my all-time favorite reagents. http://www.viddler.com/player/7964b3bb/ 31030 2009-07-09 buffer d epicentre pcr reagent review best pcr buffer pcr buffer science videos current protocols laboratory techniques benchfly molecular biology cloning biology biochemistry dna yes Alan M. http://www.benchfly.com/video/28/creating-an-aspiration-apparatus/ http://cdn-thumbs.viddler.com/thumbnail_2_6a9f642_v2.jpg Creating an aspiration apparatus No one likes a house vac full of liquid and bacteria. Set up your apparatus right to prevent the hazmat team from shutting you down... http://www.viddler.com/player/6a9f642/ 29800 2009-07-09 aspiration system primary containment secondary containment filter system tissue culture cell culture vacuum hood science videos current protocols laboratory techniques benchfly cell biology biochemistry biology cells yes Alan M. http://www.benchfly.com/video/27/nucleic-acid-absorbance-to-concentration/ http://cdn-thumbs.viddler.com/thumbnail_2_bd12908e_v2.jpg Nucleic acid absorbance to concentration Here's a quick demonstration of how to use Beer's Law to convert from nucleic acid absorbance to concentration. http://www.viddler.com/player/bd12908e/ 26940 2009-07-09 dna rna double stranded single stranded a260 absorbance concentration science videos current protocols laboratory techniques benchfly biochemistry biology molecular biology yes Alan M. http://www.benchfly.com/video/25/welcome-to-benchfly/ http://cdn-thumbs.viddler.com/thumbnail_2_518593cd_v3.jpg Welcome to BenchFly Welcome to BenchFly! Here's a brief explanation of why we started it and what BenchFly is all about. http://www.viddler.com/player/518593cd/ 48540 2009-07-09 welcome introduction greenscreen mission science videos current protocols laboratory techniques benchfly intro, yes Alan M. http://www.benchfly.com/video/24/how-to-film-a-scientific-video/ http://cdn-thumbs.viddler.com/thumbnail_2_2df222cc_v6.jpg How to film a scientific video Filming a scientific protocol is easy - here are a few suggestions to help get your film career underway! http://www.viddler.com/player/2df222cc/ 35640 2009-07-09 how to film how to shoot introduction tips advice perspective lighting sound science videos current protocols laboratory techniques benchfly tip protocol yes Alan M.