16 Search Results for "tips"

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How to Bomb-load Capillary Tips or Frits for LC/MS

https://benchfly.com/video/104/how-to-bomb-load-capillary-tips-or-frits-for-/

This is how we prepare and bomb load capillary tips and frits for use in LC/MS. Be sure to wear proper protection as this protocol uses high pressure to pack the LC/MS tips.

Tagged as: lc-ms, lcms, laboratory techniques, science videos, current protocols, proteomics,

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Timesaving Miniprep Tips

https://benchfly.com/video/31/timesaving-miniprep-tips/

Purifying DNA from dozens of bacterial samples can be painfully boring, but knowing a few timesaving miniprep tips will save your sanity...and your pipetting thumb.

Tagged as: miniprep, eppi rack, vacuum, manifold, qiagen, science videos, current protocols, laboratory techniques, benchfly, biochemistry, biology, molecular biology, dna, bacteria, plasmid,

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Estimating Volume in Pipet Tips

https://benchfly.com/video/156/estimating-volume-in-pipet-tips/

Estimating volume in pipet tips visually is an important instinct to develop. A quick visual inspection of the tip before adding to the experiment can save you from accidentally adding 20 microliters of reagent instead of 2 microliters. Here's a guide for estimating P20 volumes.

Tagged as: laboratory techniques, science videos, current protocols, biology, biochemistry, micropipette, micropipet, pipetman, molecular biology

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Important Tips for Working with Q5 Polymerase

https://benchfly.com/video/1546/important-tips-for-working-with-q5-polymerase/

Are you using Q5 High-Fidelity DNA Polymerase? Then you probably already know how robust and accurate the DNA amplification is (>100X Taq DNA Polymerase). Melanie has some tips for ensuring optimal performance with Q5. If you haven’t tried Q5 yet, what are you waiting for?

Tagged as: molecular biology, PCR, cloning

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How to Film a Scientific Video

https://benchfly.com/video/24/how-to-film-a-scientific-video/

Learning to properly film a scientific video is an important skill for modern scientists. Recording laboratory techniques, protocols, and experiments will only become more prevalent as online tools continue to impact research. Here are a few suggestions to help get your film career underway!

Tagged as: how to film, how to shoot, introduction, tips, advice, perspective, lighting, sound, science videos, current protocols, laboratory techniques, benchfly, tip, protocol

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Removing a Stuck Centrifuge Rotor

https://benchfly.com/video/41/removing-a-stuck-centrifuge-rotor/

Here's a tool that will remove a stuck centrifuge rotor and some maintenance tips that will help prevent it from happening again.

Tagged as: preventing, troubleshooting, rotor extraction, tool, repair, instrument, laboratory techniques, current protocols, key, frozen

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Putting a Centrifuge Rotor on Properly

https://benchfly.com/video/44/putting-a-centrifuge-rotor-on-properly/

Placing the rotor in the centrifuge properly is the first step to running the instrument safely. These tips can save you a lot of money in repairs!

Tagged as: rotor pins, imbalance, damage, centrifuge, instrument, laboratory techniques, current protocols, repair

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How to Pull a Capillary Tip for LC/MS

https://benchfly.com/video/102/how-to-pull-a-capillary-tip-for-lc-ms/

This is how we pull capillary tips for LC/MS use. See attached information for our exact program settings.

Tagged as: LC-MS, LCMS, laboratory techniques, current protocols, science videos, mass spec, spectrometer, proteomics,

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How to Pour an Agarose Column

https://benchfly.com/video/731/how-to-pour-an-agarose-column/

Pouring an agarose gel may not be the most exciting protocol in the world, but it's a key technique in the lab. Here we show you how to pour highly reproducible gels for technically demanding experiments. We also give tips on shortcuts you can take when pouring routine gels, where precision isn't critical. Buying resin in bulk and packing yourself is fast and is an easy way to save money.

Tagged as: column, agarose, protein, GoldBio, DNA

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Why is My Restriction Enzyme Not Cutting DNA?

https://benchfly.com/video/1537/why-is-my-restriction-enzyme-not-cutting-dna-/

There are a number of reasons that your restriction enzyme might not digest your DNA as expected, beyond having an expired lot of endonuclease. Before you toss that tube of enzyme, let Yvette walk you through some of the most common causes of unsuccessful digestion reactions. She’ll explain the control experiments for determining whether or not your samples contain contaminants, as well as tips on how to assess the methylation status of your DNA.

Tagged as: enzyme digest, troubleshooting, molecular biology, cloning, not working

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