9 Search Results for "pcr"
PCR Buffer Review (Buffer D, Epicentre)
https://benchfly.com/video/29/pcr-buffer-review-buffer-d-epicentre/PCR buffer D has never let me down and is one of my all-time favorite reagents. It's versatility makes it a perfect reagent for almost any PCR application.
How to Perform Colony PCR
https://benchfly.com/video/57/how-to-perform-colony-pcr/Colony PCR is an easy way to rapidly screen ligation reactions for positive clones - and will save you both time and money. Once you try it, this technique will change your molecular biology life.
PCR Tube Strip Labeling Tip
https://benchfly.com/video/83/pcr-tube-strip-labeling-tip/There's not much room for labeling PCR tube strips, so here's a time-saver that makes it much easier.
Tip for Loading Many Gel Samples Quickly
https://benchfly.com/video/85/tip-for-loading-many-gel-samples-quickly/I use this technique for loading many gel samples quickly when genotyping and running gels on large numbers of samples. It makes adding the loading dye to all of them very easy.
Pouring and Running an Agarose Gel
https://benchfly.com/video/91/pouring-and-running-an-agarose-gel/Knowing how to pour and run DNA agarose gel electrophoresis is essential for many molecular biology techniques. Here I show how to pour agarose gels in two of the most common types of gel boxes.
Reusing Agarose Gels
https://benchfly.com/video/108/reusing-agarose-gels/This is how I reuse agarose gels for my genotyping since I run large numbers of samples every few days - saving me time and money.
Tip for Working With Small Volumes
https://benchfly.com/video/155/tip-for-working-with-small-volumes/When adding small volumes to reactions, it's easy to question whether the liquid was actually added or not. Here's a tip that will let you rest assured that everything was setup properly.
Blue-White Screening Protocol
https://benchfly.com/video/1718/blue-white-screening-protocol/Blue-white screening based on the LacZ operon is a very powerful tool that can be used to rapidly screen bacterial colonies for clones containing your plasmid of interest. Here's a demonstration of how we perform blue-white screening at GoldBio.
Important Tips for Working with Q5 Polymerase
https://benchfly.com/video/1546/important-tips-for-working-with-q5-polymerase/Are you using Q5 High-Fidelity DNA Polymerase? Then you probably already know how robust and accurate the DNA amplification is (>100X Taq DNA Polymerase). Melanie has some tips for ensuring optimal performance with Q5. If you haven’t tried Q5 yet, what are you waiting for?
